AGROBACTERIUM-TUMEFACIENS-MEDIATED GENE-TRANSFER IN PEANUT (ARACHIS-HYPOGAEA L)

Transgenic peanut plants were produced using Agrobacterium mediated ge ne transfer. Primary leaf explants of peanut were co-cultivated with A grobacterium tumefaciens LBA 4404 harbouring the binary plasmid pBI 12 1 (conferring beta-glucuronidase activity and resistance to kanamycin) and cultured on regeneration medium supplemented with kanamycin to se lect putatively transformed shoots. They were rooted and plants were t ransferred to soil. Stable integration and expression of the transgene s were confirmed by NPT II assay, Southern blot hybridization and GUS assay.