INFLUENCE OF MEDIUM AND COLD PRETREATMENT ON ANDROGENETIC RESPONSE INLOLIUM-PERENNE L

Genotypes of Lolium perenne L. with different androgenetic responses w ere used to test effects of induction medium composition. The media te sted were potato II (pII), 190-2, and modified Linsmaier and Skoog med ia, LS-1, LS-2, and LS-3. The effect of different gelling agents, acti vated charcoal in a 'double layer' design, and casein hydrolysate were also studied. From 36,696 anthers, 25,906 embryo-like structures, 1,9 59 albino and 173 green plants were generated. Significant differences were found between media, genotypes and medium-genotype interactions studied. All three media commonly used, pII, 190-2, and LS-3, were equ ivalent in production of green plants. Cold pretreatment of the anther s (4 degrees C) significantly increased the number of embryo-like stru ctures, the number and proportion of albino plants produced, but not t he production of green plants.