Chloroplast DNA restriction fragment length polymorphisms have been us
ed to reconstruct the maternal phylogeny of all the known taxa in the
small neotropical legume genus Leucaena. Three major plastome clades w
ere recognized, but these did not conform with relationships between t
he taxa proposed on other characters from morphology, cytology or hybr
idization. The maternal parentage of tetraploids within the genus has
been proposed. Evidence for introgression was found between ''diploid'
' L. diversifolia and ''tetraploid'' L. diversifolia. The implications
of these results for the origin of the cultivated taxa are discussed.