A NOVEL TECHNIQUE OF EVALUATING FREE-RADI CAL STATUS OF PHAGOCYTING CELLS IN THE BLOOD OF PATIENTS WITH HEMOGLOBINOPATHY

Citation
Av. Bizyukin et al., A NOVEL TECHNIQUE OF EVALUATING FREE-RADI CAL STATUS OF PHAGOCYTING CELLS IN THE BLOOD OF PATIENTS WITH HEMOGLOBINOPATHY, Gematologia i transfuziologia, 39(2), 1994, pp. 41-46
Citations number
NO
Categorie Soggetti
Hematology
ISSN journal
02345730
Volume
39
Issue
2
Year of publication
1994
Pages
41 - 46
Database
ISI
SICI code
0234-5730(1994)39:2<41:ANTOEF>2.0.ZU;2-L
Abstract
Vital fluorochrome hydroethydine was used for measuring relative extra - and intracellular production of active oxygen in phagocyting blood c ells in hemoglobinopathies. Hydroethydine sensitivity to diverse free oxygen forms was studied in various model systems. Probability of fals e-positive results was estimated. It was established that hydroethydin e interacts with superoxide anion radicals in molar ratio 1:1, that in tracellular production of active oxygen forms in leukocytic mass cells stimulated by phorbolmyristatacetate was enhanced in patients with ma jor beta+-thalassemia and in a patient with hemoglobinopathy due to un stable hemoglobin Hb-Alesha. In thalassemia minor no significant diffe rences compared to control were reported. Potential causes and value o f the results obtained are under discussion.