NEURITE OUTGROWTH OF PC12 CELLS IS SUPPRESSED BY WORTMANNIN, A SPECIFIC INHIBITOR OF PHOSPHATIDYLINOSITOL 3-KINASE

Citation
K. Kimura et al., NEURITE OUTGROWTH OF PC12 CELLS IS SUPPRESSED BY WORTMANNIN, A SPECIFIC INHIBITOR OF PHOSPHATIDYLINOSITOL 3-KINASE, The Journal of biological chemistry, 269(29), 1994, pp. 18961-18967
Citations number
74
Categorie Soggetti
Biology
ISSN journal
00219258
Volume
269
Issue
29
Year of publication
1994
Pages
18961 - 18967
Database
ISI
SICI code
0021-9258(1994)269:29<18961:NOOPCI>2.0.ZU;2-Y
Abstract
The effects of wortmannin (WT), an inhibitor of phosphatidylinositol ( PI) 3-kinase, on differentiation of PC12 cells were analyzed. WT inhib ited PI 3-kinase activity of PC12 cells at a concentration of 10(-7) M in vivo and in vitro. Transient inhibition of PI 3-kinase activity at the time of nerve growth factor stimulation had no effect on activati on of the ras protein or neurite formation by the cells. However, cont inuous inhibition of PI 3-kinase blocked differentiation at the step j ust before neurite formation. When WT was applied to cells growing neu rites, elongation of the neurites was stopped at that step. These resu lts suggest that PI 3-kinase may be involved in neurite elongation.