EXPRESSION OF THE LACTOBACILLUS-CASEI LACTATE-DEHYDROGENASE GENE IN BACILLUS-SUBTILIS

Authors
KIM SMF BAEK SJ PACK MY
Citation
Smf. Kim et al., EXPRESSION OF THE LACTOBACILLUS-CASEI LACTATE-DEHYDROGENASE GENE IN BACILLUS-SUBTILIS, Biotechnology letters, 16(6), 1994, pp. 561-564
Citations number
18
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
16
Issue
6
Year of publication
1994
Pages
561 - 564
Database
ISI
SICI code
0141-5492(1994)16:6<561:EOTLLG>2.0.ZU;2-5
Abstract
A gene for allosteric lactate dehydrogenase (LDH) of Lactobacillus cas ei ATCC393 was transferred into Bacillus subtilis. The LDH was produce d in a growth-associated type, and comprised up to 40 % of the total c ellular protein. The maximum specific activity in the transformant was 208 U/mg protein which was approximately 16 times higher than in L. c asei or in the previously constructed Escherichia coli transformant.