PEROXIDASE PRODUCTION BY CARBON AND NITROGEN-SOURCES FED-BATCH CULTURE OF ARTHROMYCES-RAMOSUS

Carbon and nitrogen sources were investigated for improving peroxidase production by Arthromyces ramosus, a hyperproducer of peroxidase. Glu cose as carbon source and a mixture of yeast extract and polypeptone a t the ratio of 3 to 5 as nitrogen source in a production medium were s hown to give the highest peroxidase activity. During the culture amino acids such as alanine, arginine, methionine, leucine, tyrosine and tr yptophan were depleted. Therefore, glucose: supplemented nitrogen sour ce fed-batch culture was carried out and a peroxidase activity of 73 U /ml was obtained. This activity was 1.7 times higher than that of gluc ose fed-batch culture. This indicates that an adequate nitrogen source supply during the culture is effective for improving the peroxidase p roduction by A. ramosus.