ACCUMULATION OF CADMIUM BY HAIRY-ROOT CULTURES OF SOLANUM-NIGRUM

Cadmium uptake by cultures of transformed hairy-roots of Solanum nigru m was studied. The effect of pH, buffer type, temperature, exposure ti me and Cd2+ content ranging from 0.2 to 2000 ppm was measured. Cd2+ up take was dependent on increasing metal concentration and it was time d ependent. From the variety of buffers tested, MES buffer and berate io ns were found to be beneficial for the Cd2+ uptake. The high effectivn ess of Cd2+ accumulation in the roots decreased significantly after in creasing the Cd2+ content in the buffer over 2 ppm.