A COMPARISON OF SERIAL PLATE AGAR DILUTION, BAUER-KIRBY DISK DIFFUSION, AND THE VITEK AUTOMICROBIC SYSTEM FOR THE DETERMINATION OF SUSCEPTIBILITIES OF KLEBSIELLA SPP, ENTEROBACTER SPP, AND PSEUDOMONAS-AERUGINOSA TO 10 ANTIMICROBIAL AGENTS

Citation
T. Fekete et al., A COMPARISON OF SERIAL PLATE AGAR DILUTION, BAUER-KIRBY DISK DIFFUSION, AND THE VITEK AUTOMICROBIC SYSTEM FOR THE DETERMINATION OF SUSCEPTIBILITIES OF KLEBSIELLA SPP, ENTEROBACTER SPP, AND PSEUDOMONAS-AERUGINOSA TO 10 ANTIMICROBIAL AGENTS, Diagnostic microbiology and infectious disease, 18(4), 1994, pp. 251-258
Citations number
21
Categorie Soggetti
Microbiology,"Infectious Diseases
ISSN journal
07328893
Volume
18
Issue
4
Year of publication
1994
Pages
251 - 258
Database
ISI
SICI code
0732-8893(1994)18:4<251:ACOSPA>2.0.ZU;2-F
Abstract
The use of rapid, automated technologies for assessment of antimicrobi al susceptibility and determination of minimum inhibitory concentratio ns has been evolving for over a decade. We compared the Vitek AutoMicr obic system and Bauer-Kirby disk diffusion with the National Committee for Clinical Laboratory Standards methods of serial plate agar diluti on for qualitative and quantitative susceptibilities of 301 hospital i solates of Klebsiella spp., Enterobacter spp., and Pseudomonas aerugin osa. Antibiotics tested were aztreonam, cefoperazone, ceftazidime, cef triaxone, ciprofloxacin, gentamicin, imipenem, piperacillin, ticarcill in-clavulanic acid, and tobramycin. Agar dilution and Bauer-Kirby resu lts were more strongly correlated for all three genera than were the r esults for agar dilution and Vitek. If agar dilution is presumed to be the ''gold standard,'' Bauer-Kirby disk diffusion had only half the n umber of false susceptibles as did the Vitek. Thus, the Vitek AutoMicr obic system seems to be somewhat less reliable for both qualitative an d quantitative measurement of susceptibility and resistance than is Ba uer-Kirby disk diffusion.