Transmission electron microscopy was utilized to study the microstruct
ure of frankfurters extended with wheat germ protein flour (WGPF) at 3
.5, 5.0, and 7.0%. Samples with 3.5% WGPF showed a denser matrix than
the control. They also had a uniform interfacial protein film (IPF) wi
th a slight increase in average thickness. At WGPF >3.5%, no further e
ffects on IPF were observed. At 7.0% WGPF, an increase in added water,
and the resultant dilution of meat and fat components, resulted in fe
wer fat globules, some with incomplete IPFs. Proteins in the WGPF cont
ributed to IPF formation up to a specific level. Nonprotein components
also were important in batter stability by absorbing water.