A CHROMOSOME PAINTING METHOD FOR HUMAN SPERM CHROMOSOMES USING FLUORESCENT IN-SITU HYBRIDIZATION

Authors
CUI X TATENO H HAYATA I SATO K KAMIGUCHI Y
Citation
X. Cui et al., A CHROMOSOME PAINTING METHOD FOR HUMAN SPERM CHROMOSOMES USING FLUORESCENT IN-SITU HYBRIDIZATION, JPN J HUM G, 39(2), 1994, pp. 255-258
Citations number
5
Categorie Soggetti
Genetics & Heredity
Journal title
Japanese journal of human genetics → ACNP
ISSN journal
09168478
Volume
39
Issue
2
Year of publication
1994
Pages
255 - 258
Database
ISI
SICI code
0916-8478(1994)39:2<255:ACPMFH>2.0.ZU;2-1
Abstract
A method of chromosome painting on human sperm chromosomes using fluor escent in situ hybridization (FISH) is introduced. Sperm chromosome sl ides were prepared after in vitro fertilization of hamster eggs with h uman spermatozoa. The slides were treated by RNase A before FISH. Chro mosome 4 was clearly and specifically painted in a majority of sperm-d erived metaphase plates after an application of whole chromosome paint ing DNA probes of this chromosome. This is the first report of success ful painting on human sperm chromosomes.