SELECTIVELY C-13-ENRICHED DNA - C-13 AND H-1 ASSIGNMENTS OF A TRIPLE-HELIX BY 2-DIMENSIONAL RELAYED HMQC EXPERIMENTS

Citation
O. Bornet et al., SELECTIVELY C-13-ENRICHED DNA - C-13 AND H-1 ASSIGNMENTS OF A TRIPLE-HELIX BY 2-DIMENSIONAL RELAYED HMQC EXPERIMENTS, Journal of biomolecular NMR, 4(4), 1994, pp. 575-580
Citations number
27
Categorie Soggetti
Biology,Spectroscopy
Journal title
ISSN journal
09252738
Volume
4
Issue
4
Year of publication
1994
Pages
575 - 580
Database
ISI
SICI code
0925-2738(1994)4:4<575:SCD-CA>2.0.ZU;2-L
Abstract
We present NMR studies of an intramolecular triple helix, the three st rands of which have been linked by a hexaethylene glycol chain. To ove rcome the generally encountered difficulties of assignment in the homo pyrimidine strands, the carbon C1' of the pyrimidines were selectively C-13-enriched. Assignments of the aromatic and sugar protons were obt ained from NOESY-HMQC and TOCSY-HMQC spectra. We show that the recogni tion of a DNA duplex by a third strand via tripler formation is easily carried out in solution by observing the changes of the (1)H1'-(13)C1 ' connectivities as a function of pH. Furthermore, the conformation of the sugars has been found to be C2'-endo, on the basis of the couplin g constant values directly measured in an HSQC spectrum.