Stem infusion was evaluated for quantifying downward movement, intrace
llular allocation, and interorgan partitioning of assimilate in sweet
and grain sorghum [Sorghum bicolor (L.) Moench] stems during mid-grain
fill and post-black layer. Sucrose concentration did not vary below t
he site of infusion with [C-14]sucrose, although gradients of intracel
lular 80%-ethanol soluble and insoluble C-14-components were establish
ed in the stem. Variation in gradient steepness between cultivars resu
lted from aspects of parenchymal uptake or phloem uptake of sucrose an
d appears related to sucrose-concentrating ability. The introduced [C-
14]sucrose concentration gradient in stem tissue is useful for studyin
g stem mechanisms that control sink strength.