INTERACTION OF AVIAN REOVIRUS WITH CHICKEN LYMPHOBLASTOID CELL-LINES

Four chicken lymphoblastoid cell lines were inoculated with avian reov irus strain S1133 and two local isolates, 965 and 615. Of the inoculat ed cell lines, TLT, a B-cell line, was productively infected with the three viruses as demonstrated by immunofluorescence assay (IFA) and ra dioimmunoprecipitation assay. A comparative growth curve analysis of t he three avian reoviruses was done at 37-degrees and 41-degrees-C. Iso late 965 replicated to a higher titre at both temperatures while the r eplication of S1133 and 615 was found to be inhibited at 41-degrees-C. IFA revealed that among the transformed T- lymphoblastoid cells used in this study, only MDCC-RP1 was permissive to virus infection with is olate 965, and at 41-degrees-C, but not 37-degrees-C.