R. Stolper et Jp. Opdebeeck, FLEA ALLERGY DERMATITIS IN DOGS DIAGNOSED BY INTRADERMAL SKIN-TESTS, Research in Veterinary Science, 57(1), 1994, pp. 21-27
Thirty-three dogs were categorised according to their clinical signs o
f flea allergy dermatitis and reactivity to feeding fleas (Ctenocephal
ides felis felis). A soluble extract of whole fleas (FS), fractions of
this extract separated by chromatography, and a commercially availabl
e flea antigen extract were used in intradermal skin tests (IDST) to e
stablish the presence or absence of type I and type IV hypersensitivit
y. The reactions were measured and the results were analysed using thr
ee grading systems commonly reported in the literature. The results of
the IDST for the groups of dogs varied according to the grading syste
m used. FS, the most effective of the antigen preparations, identified
94 per cent of dogs which reacted to feeding fleas when a result was
considered positive if the mean diameter of the wheal at the antigen i
njection site exceeded the diameter of the wheal at the negative contr
ol site by five mm at 15 and/or 30 minutes after injection.