S. Takizawa et al., LACTOBACILLUS-KEFIRGRANUM SP-NOV AND LACTOBACILLUS-PARAKEFIR SP-NOV, 2 NEW SPECIES FROM KEFIR GRAINS, International journal of systematic bacteriology, 44(3), 1994, pp. 435-439
Twelve strains of homofermentative lactobacilli and two strains of het
erofermentative lactobacilli were isolated from kefir grains by using
R-CW agar medium. The physiological and biochemical characteristics, D
NA guanine-plus-cytosine contents, and levels of DNA-DNA relatedness o
f these isolates and previously described lactobacilli were compared.
Our results indicated that two new species, Lactobacillus kefirgranum
and Lactobacillus parakefir, could be distinguished. The type strain o
f L. kefirgranum sp. nov. is GCL 1701 (= JCM 8572), and the type strai
n of L. parakefir sp. nov. is GCL 1731 (= JCM 8573).