Wt. Cefalu et al., GLYCOHEMOGLOBIN MEASURED BY AUTOMATED AFFINITY HPLC CORRELATES WITH BOTH SHORT-TERM AND LONG-TERM ANTECEDENT GLYCEMIA, Clinical chemistry, 40(7), 1994, pp. 1317-1321
We evaluated glycohemoglobin (GHb) and glycated plasma protein (GPP) b
y automated affinity HPLC for their ability to monitor both short-term
and long-term antecedent glycemia in 70 diabetic subjects. We placed
30 subjects on an intervention protocol in which insulin and (or) diet
ary changes were made twice weekly to acutely decrease glycemia. We mo
nitored 40 subjects at 6-week intervals; changes in the clinical regim
en were made at that time only. Despite weekly changes in mean blood g
lucose in the subjects who received more intensive intervention, GHb c
oncentrations correlated significantly with the weekly (r = 0.66, P <0
.001), 2-week (r = 0.70, P <0.001), 3-week (r = 0.72, P <0.001), and 6
-week (r = 0.83, P <0.001) mean glucose concentrations. GPP correlated
significantly with measured glycated albumin determined by boronate a
ffinity columns (r = 0.83, P <.001) and correlated best with the 1-wee
k (r = 0.66, P <0.001), 2-week (r = 0.64, P <.001) and 3-week (r = 0.6
0, P <0.001) mean antecedent glucose concentration. Thus, GHb, traditi
onally considered a marker for only long-term diabetic control, correl
ated significantly with both shortterm and long-term antecedent glycem
ia.