GLUCOSE POTENTIOMETRIC ELECTRODES BASED ON MEDIATORLESS BIOELECTROCATALYSIS - A NEW APPROACH

A potentiometric method has been developed for analysis of glucose on the basis of glucose oxidase and peroxidase co-immobilized on the surf ace of an electrode made of composite carbonic material. Catalytic oxi dation of glucose results in the formation of hydrogen peroxide. Media torless peroxidase catalysis of electroreduction of hydrogen peroxide leads to a shift in the electrode potential. The rate of the increase of the electrode potential is proportional to glucose concentration an d calibration curve is linear at 0.025-2.0 mM. Electrodes permit at le ast 100 measurements without any loss in the activity. Electrodes rema in stable for 90 days when stored at 277 K.