OPTIMIZATION OF CULTURE CONDITIONS OF BREVIBACTERIUM SP FOR THE PRODUCTION OF AMIDASE AND ADIPAMIDASE

The culture conditions required for the maximum production of the wide spectrum amidase and the adipamidase were determined for two strains of Brevibacterium sp. applying. two mathematical techniques: the Hadam ard matrix and the analyses of response surface. A culture medium was optimized and the role of Zn2+ on the activity of adipamidase was demo nstrated. The enzyme was shown to be not subject to catabolic repressi on by glucose or ammonium sulfate and it was not inducible. The influe nce of physical and chemical factors (pH, temperature) on cell growth and enzyme activity was also studied.