MONOCLONAL-ANTIBODIES THAT RECOGNIZE THE TYPE-2 ACTIVIN RECEPTOR, ACTR2

Monoclonal antibodies (MAbs) were raised in mice against a bacterial f usion protein composed of the intracellular serine/threonine kinase do main of the type-2 activin receptor, ACTR2, fused to glutathione S-tra nsferase. Three MAbs with high affinity toward the ACTR2 kinase domain were isolated, one of which recognized specifically ACTR2 expressed t ransiently in vascular endothelial cells. These reagents should be of use in the elucidation of mechanisms of transmembrane signaling by thi s member of the emerging receptor serine threonine kinase family.