E. Horvath et al., P-32 POST-LABELING DETECTION OF DNA-ADDUCTS IN MONONUCLEAR-CELLS OF WORKERS OCCUPATIONALLY EXPOSED TO STYRENE, Carcinogenesis, 15(7), 1994, pp. 1309-1315
P-32-Post-labeling was used to analyze for the presence of DNA adducts
in 47 workers exposed to styrene in a boat manufacturing facility. In
dividual airborne exposures measured several times over the course of
1 year ranged from 1 to 235 mg/m(3) with a mean value of 65.6 mg/m(3).
Two adducts were detected in the DNA of mononuclear cells of these wo
rkers. The following levels of adducts were detected: adduct 1, range
0.6-102 x 10(-8) (mean 15.8 x 10(-8)); adduct 2, range 0.1-70.9x10(-8)
(mean 14.2x10(-8)). Significant linear relationships were found betwe
en styrene exposure and both DNA adducts (adduct 2, r = 0.330, P = 0.0
12; adduct 1, r = 0.244, P = 0.049). Co-chromatography experiments ide
ntified DNA adduct 1 in the exposed samples as phenylethyl)-2'-deoxygu
anosine-3',5'-bisphosphate. DNA adduct 2 remains unidentified. No sign
ificant linear relationships were observed between the level of DNA ad
ducts and sister chromatid exchanges, possibly because of the poor pre
cision of the P-32-post-labeling assay (the estimated coefficients of
variation for adducts 1 and 2 were 2.54 and 1.96, respectively). These
results demonstrate that occupational exposure to styrene results in
the formation of DNA adducts in human mononuclear cells.