KLUYVEROMYCES-MARXIANUS - A POTENTIAL SOURCE OF DIACETYL REDUCTASE

Citation
Jg. Schwarz et Yd. Hang, KLUYVEROMYCES-MARXIANUS - A POTENTIAL SOURCE OF DIACETYL REDUCTASE, World journal of microbiology & biotechnology, 10(4), 1994, pp. 385-387
Citations number
15
Categorie Soggetti
Biothechnology & Applied Migrobiology
ISSN journal
09593993
Volume
10
Issue
4
Year of publication
1994
Pages
385 - 387
Database
ISI
SICI code
0959-3993(1994)10:4<385:K-APSO>2.0.ZU;2-1
Abstract
Kluyveromyces marxianus had a higher specific activity of diacetyl red uctase (EC 1.1.1.5) than all other organisms previously reported. The enzyme was NADH-dependent and irreversibly catalysed the conversion of diacetyl to acetoin with an optimum pH of 7.0. It was stable at 40-de grees-C but lost 50% of its activity at 50-degrees-C in 30 min. The K( m) and V(max) values for diacetyl were 1.8 mm and 0.053 mm/m in, respe ctively.