GENERATION AND CHARACTERIZATION OF A HUMAN MONOCLONAL IGG ANTIBODY SPECIFIC FOR HLA-B12

Authors
SIEMONEIT K WOLPL A WEGENER S ERNST M SONNEBORN HH GOLDMANN SF
Citation
K. Siemoneit et al., GENERATION AND CHARACTERIZATION OF A HUMAN MONOCLONAL IGG ANTIBODY SPECIFIC FOR HLA-B12, Tissue antigens, 44(1), 1994, pp. 25-33
Citations number
36
Categorie Soggetti
Immunology,"Cytology & Histology
Journal title
Tissue antigens → ACNP
ISSN journal
00012815
Volume
44
Issue
1
Year of publication
1994
Pages
25 - 33
Database
ISI
SICI code
0001-2815(1994)44:1<25:GACOAH>2.0.ZU;2-F
Abstract
We describe here the generation and characterization of a human monocl onal IgG antibody (UL/F14) specific for HLA-B12. The antibody is suita ble for complement-dependent lysis on lymphoblastoid cell lines and st imulated peripheral blood mononuclear cells. UL/F14 competes for antig en binding with an HLA-B12 human monoclonal IgM antibody and with a sp ecific alloantiserum. Protein chemistry shows that the monoclonal anti body UL/F14 can precipitate solubilized HLA-B12 molecules.