A NOVEL METHOD FOR DETECTING ACETYLGLUCOSAMINIDASE ACTIVITY ON POLYACRYLAMIDE GELS

Authors
LINCOLN SP FERMOR TR WOOD DA
Citation
Sp. Lincoln et al., A NOVEL METHOD FOR DETECTING ACETYLGLUCOSAMINIDASE ACTIVITY ON POLYACRYLAMIDE GELS, Journal of microbiological methods, 20(2), 1994, pp. 79-85
Citations number
9
Categorie Soggetti
Microbiology,"Biochemical Research Methods
Journal title
Journal of microbiological methods → ACNP
ISSN journal
01677012
Volume
20
Issue
2
Year of publication
1994
Pages
79 - 85
Database
ISI
SICI code
0167-7012(1994)20:2<79:ANMFDA>2.0.ZU;2-C
Abstract
Acetylglucosaminidase (EC 3.2.1.30) activity could be detected after S DS PAGE, non-SDS PAGE and isoelectric focusing. After SDS PAGE, enzyme activity was restored by washing in Triton X-100. Detection of acetyl glucosaminidase bands was by incorporating in the gel, or incubation w ith, 4-methylumbelliferyl N-acetyl-beta-D-glucosaminide.