IMPROVED RAPID METHOD FOR PRODUCTION AND PURIFICATION OF CLOSTRIDIUM-PERFRINGENS TYPE-A ENTEROTOXIN

Authors
HEREDIA NL GARCIAALVARADO JS LABBE RG
Citation
Nl. Heredia et al., IMPROVED RAPID METHOD FOR PRODUCTION AND PURIFICATION OF CLOSTRIDIUM-PERFRINGENS TYPE-A ENTEROTOXIN, Journal of microbiological methods, 20(2), 1994, pp. 87-91
Citations number
22
Categorie Soggetti
Microbiology,"Biochemical Research Methods
Journal title
Journal of microbiological methods → ACNP
ISSN journal
01677012
Volume
20
Issue
2
Year of publication
1994
Pages
87 - 91
Database
ISI
SICI code
0167-7012(1994)20:2<87:IRMFPA>2.0.ZU;2-G
Abstract
Clostridium perfringens strain FD-1041 was grown in Duncan-Strong medi um containing raffinose for 5 h at 43 degrees C. Enterotoxin in the ce ll extract was purified 5.5-fold by a modified ammonium sulfate-gel fi ltration method using Sephacryl S-200. The enterotoxin could be purifi ed to homogeneity with 50% recovery in less than 48 h.