Ss. Marwaha et al., OPTIMIZATION OF PARAMETERS FOR HYDROLYSIS OF LIMONIN FOR DEBITTERING OF KINNOW MANDARINE JUICE BY RHODOCOCCUS FASCIANS, Enzyme and microbial technology, 16(8), 1994, pp. 723-725
The use of Rhodococcus fascians for debittering kinnow juice was explo
red. Its growth and application conditions were optimized. Incubation
of kinnow juice with 5% (v/v) R. fascians mass cultivated for 48 h aff
orded optimal (60%) degradation of limonin at 25 degrees C, pH 4.0 and
150 rev min-(1) for 48 h. Coupling of this step with alkaline degrada
tion led to 78% degradation of limonin.