Taking advantage of the sensitivity, the lateral resolution and the ab
ility of secondary ion mass spectrometry (SIMS) for elemental imaging,
we have shown that the main alkaline and all;aline-earth cations exhi
bited clear-cut regionalization in flax seeds. Calcium, and even more
magnesium were almost exclusively located within the protein bodies (P
Bs); and they were practically absent from the tissue areas with no PB
s, such as the provascular tissue. By contrast, sodium was almost excl
usively located outside the PBs; moreover, most of the seed sodium was
in the tissues at the seed periphery (chiefly the teguments). Among t
he studied cations, potassium was the only one with a non-negligible c
oncentration both in and outside the PBs.