CLONING OF THE MACROLIDE ANTIBIOTIC BIOSYNTHESIS GENE ACYA, WHICH ENCODES 3-O-ACYLTRANSFERASE, FROM STREPTOMYCES THERMOTOLERANS AND ITS USEFOR DIRECT FERMENTATIVE PRODUCTION OF A HYBRID MACROLIDE ANTIBIOTIC
A. Arisawa et al., CLONING OF THE MACROLIDE ANTIBIOTIC BIOSYNTHESIS GENE ACYA, WHICH ENCODES 3-O-ACYLTRANSFERASE, FROM STREPTOMYCES THERMOTOLERANS AND ITS USEFOR DIRECT FERMENTATIVE PRODUCTION OF A HYBRID MACROLIDE ANTIBIOTIC, Applied and environmental microbiology, 60(7), 1994, pp. 2657-2660
A gene encoding the macrolide modification enzyme 3-O-acyltransferase
(acyA) was cloned by chromosome walking onto the carbomycin biosynthet
ic region in Streptomyces thermotolerans TH475, with the 3' region of
the gene encoding the macrolide modification enzyme 4''-O-acyltransfer
ase (acyB1) as a probe. A shortened fragment (1.8 kb) containing acyA
was subcloned with pIJ350. A high level tylosin producer, Streptomyces
fradiae MBBF, transformed with the plasmid could produce a hybrid mac
rolide, 3-O-acetyltylosin, most efficiently.