T. Rui et al., PROTECTIVE EFFECT OF CAPTOPRIL ON CULTURE D RAT MYOCARDIAL-CELLS WITHANOXIA AND REOXYGENATION INJURY, Zhongguo yaoli xuebao, 15(4), 1994, pp. 375-378
The effect of captopril (Cap) on electric activity of cultured rat myo
cardial cells under anoxia and reoxygenation was studied with standard
microelectrode techinques. Results showed that anoxic solution caused
lowerings of MDP, APA, and V-max, and a shortening of APD(50). All my
ocytes revealed multiform arrhythmias, and most cells stopped beating
within 30 min, while only 40 % of the cells exhibited arrhythmias but
none stopped beating-in the presence of 40 mg . L(-1) under the same c
ondition. During reoxygenation, most cells resumed beating in 10 min b
ut some of these cells stopped beating again. The electric activities
in rebeating cells during reoxygenation for 30 min were lower than tho
se in normoxic cells. Cap (40 mg . L(-1))-treated cells reheat quickly
after reoxygenation and no cell stopped beating any more, with parame
ters higher than those in untreated cells. These results demonstrate t
hat Cap yields some beneficial effects on preventing anoxia and reoxyg
enation injury in cultured rat myocardial cells.