By use of a degenerate oligodeoxyribonucleotide probe corresponding to
the N-terminal amino acid (aa) sequence and Southern blot analysis, t
he gene encoding the di-heme cytochrome c(4) (CC4) from Pseudomonas st
utzeri has been cloned. The aa sequence deduced from the nucleotide se
quence shows a 20-aa signal peptide and a 190-aa mature protein with 7
9% identity to the Azotobacter vinelandii CC4 sequence, which had earl
ier been determined by aa sequencing.