KINETIC-STUDY OF DEACETYLATION AND ACETYL MIGRATION OF PERACETYLATED 1-METHYL ALPHA,BETA-D-GLUCOPYRANOSIDES BY CANDIDA LIPASE-CATALYZED HYDROLYSIS

Authors
HSIAO KF LIN HJ LEU DL WU SH WANG KT
Citation
Kf. Hsiao et al., KINETIC-STUDY OF DEACETYLATION AND ACETYL MIGRATION OF PERACETYLATED 1-METHYL ALPHA,BETA-D-GLUCOPYRANOSIDES BY CANDIDA LIPASE-CATALYZED HYDROLYSIS, Bioorganic & medicinal chemistry letters, 4(13), 1994, pp. 1629-1632
Citations number
8
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
4
Issue
13
Year of publication
1994
Pages
1629 - 1632
Database
ISI
SICI code
0960-894X(1994)4:13<1629:KODAAM>2.0.ZU;2-F
Abstract
Peracetylated 1-methyl alpha,beta-D-glucopyranosides (1 and 2) were hy drolyzed by Candida lipase and 6-OH, 4-OH and 4,6-OH derivatives were obtained. A kinetic study revealed that the reaction was a consecutive irreversible three-stage reaction including one acetyl migration and two enzymatic deacetylation reactions. The three rate constants were m easured and theoretical profiles for predicting the amount of products and substrates against reaction time were determined.