RECOVERY OF ANTIBIOTICS BY AQUEOUS 2-PHASE PARTITION - PARTITIONING BEHAVIOR OF PURE ACETYLSPIRAMYCIN SOLUTION IN POLYETHYLENE-GLYCOL POTASSIUM PHOSPHATE AQUEOUS 2-PHASE SYSTEMS

Authors
GUAN YX MEI LH ZHU ZQ
Citation
Yx. Guan et al., RECOVERY OF ANTIBIOTICS BY AQUEOUS 2-PHASE PARTITION - PARTITIONING BEHAVIOR OF PURE ACETYLSPIRAMYCIN SOLUTION IN POLYETHYLENE-GLYCOL POTASSIUM PHOSPHATE AQUEOUS 2-PHASE SYSTEMS, Biotechnology techniques, 8(7), 1994, pp. 491-496
Citations number
7
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
8
Issue
7
Year of publication
1994
Pages
491 - 496
Database
ISI
SICI code
0951-208X(1994)8:7<491:ROABA2>2.0.ZU;2-I
Abstract
The effects of average molecular weight of PEG, concentrations of PEG and KH2PO4 and pH on the partition equilibrium of acetylspiramycin in PEG/KH2PO4 aqueous two-phase systems were studied in detail. The parti tion coefficients of acetylspiramycin in PEG/KH2PO4 systems were measu red at room temperature 25-degrees-C. It was found that acetylspiramyc in partitioned unevenly in the aqueous two-phase systems composed of P EG and KH2PO4 and could be purified by this technique. A suitable phas e-forming system (pH=6.7, 12w/w% PEG2000, 11w/w% KH2PO4) was found out after partition coefficient (Kp=42), extraction ratio (eta=96%) and r ecovery ratio(R=98.8%) were investigated comprehensively in this paper .