MYELOID ACTIVATION ANTIGEN CD66 67 - IMMUNOCHEMICAL AND IMMUNOCYTOMETRIC CHARACTERIZATION WITH THE 5TH WORKSHOP MONOCLONAL-ANTIBODIES/

Flow cytometric and immunochemical studies performed with the CD66/67 panel antibodies from the Vth International Workshop on Leukocyte Anti gens allowed to subdivide these antibodies into five groups, according to the molecular weights of polypeptides recognized by these antibodi es on pooled healthy donor granulocytes. Although some monoclonal anti bodies recognized either 95-110 kDa, or 160-180 kDa polypeptides, majo rity of the examined antibodies reacted with epitopes shared by both 9 5-110 kDa and 160-180 kDa polypeptides. Differential TPA-induced modul ation of recognized antigens was observed with HL-60 and U-937 leukemi a/lymphoma cell lines.