PIG INTESTINAL MEMBRANE-BOUND RECEPTOR (GUANYLYL CYCLASE) FOR HEAT-STABLE ENTEROTOXIN - CDNA CLONING, FUNCTIONAL EXPRESSION, AND CHARACTERIZATION

Authors
WADA A HIRAYAMA T KITAO S FUJISAWA J HIDAKA Y SHIMONISHI Y
Citation
A. Wada et al., PIG INTESTINAL MEMBRANE-BOUND RECEPTOR (GUANYLYL CYCLASE) FOR HEAT-STABLE ENTEROTOXIN - CDNA CLONING, FUNCTIONAL EXPRESSION, AND CHARACTERIZATION, Microbiology and immunology, 38(7), 1994, pp. 535-541
Citations number
23
Categorie Soggetti
Microbiology,Immunology
Journal title
Microbiology and immunology → ACNP
ISSN journal
03855600
Volume
38
Issue
7
Year of publication
1994
Pages
535 - 541
Database
ISI
SICI code
0385-5600(1994)38:7<535:PIMR(C>2.0.ZU;2-#
Abstract
A cDNA encoding the receptor protein for a heat-stable enterotoxin (ST a) produced by enterotoxigenic Eschrerichia coli was cloned from intes tinal epithelial cells of a 10-week-old pig. The cDNA had an open read ing frame of 3,219 base pairs and coded for a protein with 1,073 amino acid residues. The mature protein consisted of 1,050 amino acid resid ues with a molecular mass of ca. 121 kDa and was 87% and 82% identical with the human and rat protein, respectively. The CHO cell line overe xpressing the pig recombinant STa receptor specifically bound to a pho toaffinity-labeled analog of STa and showed marked elevation of the ce llular content of cGMP in response to STa.