EFFECTS OF FREEZE-THAW INJURY ON PARAMETERS OF DISTRIBUTED ELECTRICALCIRCUITS OF STEMS AND NEEDLES OF SCOTS PINE-SEEDLINGS AT DIFFERENT STAGES OF ACCLIMATION
T. Repo et al., EFFECTS OF FREEZE-THAW INJURY ON PARAMETERS OF DISTRIBUTED ELECTRICALCIRCUITS OF STEMS AND NEEDLES OF SCOTS PINE-SEEDLINGS AT DIFFERENT STAGES OF ACCLIMATION, Journal of Experimental Botany, 45(275), 1994, pp. 823-833
Frost hardening and frost injury of both the stems and needles of Scot
s pine (Pinus sylvestris L.) seedlings were studied by electrical impe
dance analysis. This impedance analysis was based on equivalent circui
ts with distributed circuit elements (DCE). A double-DCE model for ste
ms and two single-DCE models for needles provided excellent fit to the
experimental impedance data. However, the single-DCE model for needle
s which takes into account the asymmetry of the impedance are proved m
ore appropriate than the model. Several parameters changed in to frost
injury. In stems, extracellular resistance and one relaxation time de
creased with increasing damage, whereas intracellular resistance remai
ned relatively unchanged. In needles, the overall pattern for extracel
lular resistance and relaxation time was similar to that in the stem.
Intracellular resistance remained approximately constant in the case o
f the symmetric DCE model. During frost hardening, both extracellular
and intracellular resistance increased in stems. In needles, extracell
ular resistance increased but relaxation time decreased with hardening
. The skewness of the impedance spectra in the Cole-Cole plot for need
les increased with hardening. The coefficient for distribution of the
relaxation time(s) did not change in either stems or needles with fros
t hardening but some changes were found with frost damage.