PREDICTION OF THE CONCENTRATION OF SEVERAL CONSTITUENTS IN A MOUSE-MOUSE HYBRIDOMA CULTURE BY NEAR-INFRARED SPECTROSCOPY

Near infrared spectroscopy (NIR) was employed to predict the concentra tions of the nutrients, glucose and glutamine, and the products, lacti c acid and ammonia, in the culture broth of a mouse-mouse hybridoma. A supernatant of the culture broth was supplied to a near infrared spec trophotometer and the absorbance at wavelengths between 1,100 and 2,50 0 nm was measured. The correlations between the concentrations obtaine d by conventional methods and those obtained by NIR were examined by m ultiple regression analysis. NIR values predicted by 5-wavelength regr ession were in good agreement with those obtained by the usual methods . The multiple correlation coefficient was over 0.98 for all four subs tances. The procedure involved in NIR is simple, and the operation tim e required to predict the concentrations is only 5 min. The results su ggest that NIR is a useful method for the control of animal cell cultu res.