TOWARD A PHYSICAL MAP OF HUMAN-CHROMOSOME-10 - ISOLATION OF 183 YACS REPRESENTING 80 LOCI AND REGIONAL ASSIGNMENT OF 94 YACS BY FLUORESCENCE IN-SITU HYBRIDIZATION

One hundred eighty-three YACs carrying human chromosome 10 sequences w ere isolated from multigenome equivalent libraries by PCR-based screen ing for the presence of 80 different chromosome 10-specific STSs. Nine ty-four of the isolated YACs, representing 52 genes and DNA segments, were mapped to regions of chromosome 10 by fluorescence in situ hybrid ization. The results localized 26 DNA segments to cytogenetic bands fo r the first time. About 37% (35/94) of the YACs hybridized to more tha n one chromosomal location: 31 to other chromosomes in addition to chr omosome 10 and 4 to 2 distinct locations on chromosome 10. These resul ts are consistent with the number of chimeric YACs expected from these libraries but may also reflect the presence of 2 or more YACs within a single clone or the presence of low copy repeated elements within th e genome. This STS anchor screening effort resulted in the identificat ion of 69 contigs, with 7 contigs consisting of 2 anchors each and 1 c ontig consisting of 5 anchors. All linked STSs were multiply linked by at least 2 independent YACs. These anchored YACs span the entire chro mosome and appear to cover 15% of chromosome 10. (C) 1994 Academic Pre ss, Inc.