SEQUENCE HOMOLOGIES AND LINKAGE GROUP CONSERVATION OF THE HUMAN AND MOUSE CENPC GENES

Using a previously identified human CENPC cDNA fragment, we have isola ted cDNA clones corresponding to the complete mouse Cenpc coding seque nce. Using these cDNAs as probes to genomic libraries, we have isolate d genomic clones corresponding to the mouse and human genes and also t o a mouse pseudogene. In situ hybridization mapping of these genes rev eals that the human gene maps to 4q12-q13.3 and the mouse gene to 5E2- E5. These sites are in a region of linkage group conservation between the two species. Secondary sites are present in man on chromosome 12q2 1.2-q21.33 and in mouse on chromosome 2B. This mouse secondary site is a pseudogene on the basis of DNA sequence. These secondary sites are not syntenic in the two species. (C) 1994 Academic Press, Inc.