Jd. Wang et al., IMMUNOCYTOCHEMICAL COLOCALIZATION OF PROGESTERONE-RECEPTOR AND PROLACTIN IN INDIVIDUAL STROMAL CELLS OF HUMAN DECIDUA, The Journal of clinical endocrinology and metabolism, 79(1), 1994, pp. 293-297
Progesterone stimulates decidual PRL production. However, decidualized
tissue, in which a high level of PRL is present, features a relativel
y low level of progesterone receptor (PR) expression. The discrepancy
has to be explored at the individual cell level. The present study emp
loyed a double labeling method to colocalize PR and PRL in human decid
ua to examine the correlation between these two proteins. In frozen se
ctions, decidual stroma presented two kinds of cell, which differed in
PRL staining. Decidualized cells were positively stained with PRL in
cytoplasm and displayed a large cell size and a clear cell outline. No
ndecidualized cells showed no specific PRL staining and no dear cell b
oundary. In decidual stroma, PR staining was exclusively localized in
the nuclei, with variations in intensity. When double staining with PR
and PRL was performed, these two types of cells demonstrated diverse
staining patterns. The PRL-producing cells exhibited weak PR staining,
whereas PRL-negative cells evidenced stronger PR staining. In RU 486-
treated samples, decidual stroma became less stained with PRL, compare
d with the control, and fewer cells displayed typical morphology of de
cidualization, whereas PR staining in the tissue became more extensive
and intensive. Double labeling disclosed that the cells with enhanced
PR staining were coupled to weaker PRL immunoreaction. Our data sugge
sted an inverse relationship between PRL and PR in individual stromal
cells in vivo, which could be reversed by antiprogestin treatment. A p
ossible autocrine mechanism controlling this phenomenon was proposed a
nd deserves further study.