THE EXISTENCE OF PROTEIN-KINASE-C IN CONE PHOTORECEPTORS IN THE RAT RETINA

We examined the localization of protein kinase C (PKC) in the rat reti na, using a monoclonal antibody against PKC. The PKC immunoreactivitie s were localized in bipolar cells as reported previously, and also in outer and inner segments (OS and IS) of photoreceptor cells. As the PK C immunoreactive OS and IS of photoreceptors were very few in number, we hypothesized that these were of cone photoreceptors. We then examin ed whether the PKC immunoreactive OS and IS were bound by peanut agglu tinin which was shown to bind specifically to cone OS and IS. Almost a ll of the PKC immunoreactive OS and IS showed PNA binding.