A MONOCLONAL INHIBITION ENZYME-IMMUNOASSAY FOR DETECTION OF ANTIBODIES AGAINST HEPATITIS-B CORE ANTIGEN - CONFIRMATION OF AN IMMUNODOMINANTEPITOPE

Authors
PUJOL FH BERTOLOTTI A FIELDS HA KHUDYAKOV YE KALININA TI LIPRANDI F
Citation
Fh. Pujol et al., A MONOCLONAL INHIBITION ENZYME-IMMUNOASSAY FOR DETECTION OF ANTIBODIES AGAINST HEPATITIS-B CORE ANTIGEN - CONFIRMATION OF AN IMMUNODOMINANTEPITOPE, Journal of immunoassay, 15(3), 1994, pp. 239-249
Citations number
22
Categorie Soggetti
Immunology
Journal title
Journal of immunoassay → ACNP
ISSN journal
01971522
Volume
15
Issue
3
Year of publication
1994
Pages
239 - 249
Database
ISI
SICI code
0197-1522(1994)15:3<239:AMIEFD>2.0.ZU;2-5
Abstract
Monoclonal antibodies (mAbs) were raised against hepatitis B virus cor e produced by a recombinant clone of Escherichia coli (rHBc). The thre e mAbs recognized rHBc by Western blot, suggesting that they reacted w ith non-conformational epitopes. Competition experiments between mAbs and human anti-HBc sera confirmed the existence of an immunodominant H Bc epitope within the viral antigen. A monoclonal competition enzyme i mmunoassay using an IgM mAb conjugated to biotin and streptavidin-pero xidase as the detection system yielded 99% sensitivity and 100% specif icity, when compared to other commercial assays.