CORRELATION BETWEEN INFLAMMATORY CELLULAR-RESPONSES AND CHEMOTACTIC ACTIVITY IN BRONCHOALVEOLAR LAVAGE FLUID FOLLOWING INTRATRACHEAL INSTILLATION OF NICKEL SULFATE IN RATS
S. Hirano et al., CORRELATION BETWEEN INFLAMMATORY CELLULAR-RESPONSES AND CHEMOTACTIC ACTIVITY IN BRONCHOALVEOLAR LAVAGE FLUID FOLLOWING INTRATRACHEAL INSTILLATION OF NICKEL SULFATE IN RATS, Archives of toxicology, 68(7), 1994, pp. 444-449
In a preceding study, we reported that the numbers of macrophages and
polymorphonuclear leukocytes (PMN) were increased in bronchoalveolar l
avage fluid (BALF) following the intratracheal instillation of nickel
sulfate (NiSO4) in rats. In the present study, BALF chemotactic activi
ties for both macrophages and PMN were measured to investigate if the
increases of these inflammatory cells in BALF depend on increases in c
hemotactic activities in epithelial lining fluid (ELF) of the lung. Bo
th the number of PMN and the PMN chemotactic activity peaked at 2 days
post-instillation and they were significantly correlated. However, th
e PMN chemotactic activity was inversely correlated with concentration
of leukotriene B-4 (LTB(4)), a well-known chemotaxin. Although PMN we
re not observed in control BALE LTB(4) concentration in the control EL
F (ca. 5 x 10(-7) M) was estimated to have a potential to attract PMN
chemotactically through a membrane in in vitro migration assay. These
results suggest that the presence of LTB(4) in ELF itself does not tri
gger transpulmonary PMN infiltration. The rat BALE were fractionated b
y high performance liquid chromatography (HPLC), and PMN chemotactic a
ctivity of each fraction was measured. The elution profiles of PMN che
motactic activity showed that there were at least two different chemot
axins in BALF obtained from the NiSO4-exposed rats. Macrophage chemota
ctic activity in BALF also peaked at 2 days post-instillation. However
, the number of macrophages was not significantly correlated with the
chemotactic activity for macrophage in BALE The HPLC study showed that
the macrophage chemotactic substance in the BALE obtained from NiSO4-
exposed rats was different from complement fragment (C5a) and its MW w
as estimated to be 10-12 kD.