Ml. Paine et al., NON-COEXPRESSION OF COLLAGENASE AND TRANSIN IN MALIGNANT EPITHELIAL-CELLS, International journal of oncology, 10(3), 1997, pp. 615-618
Increased synthesis and secretion of the matrix metalloproteinase has
been associated with the acquisition of the invasive properties of mal
ignant cells. In a rat malignant anaplastic cell line (T952/F7) sporad
ic expression of mRNA for collagenase-3 against a background of high t
ransin-l mRNA has been shown. Sporadic induction of either metalloprot
einase was not found in the benign precursor cells (A5P/B10) of T952/F
7 cells, but a coordinate mRNA induction profile for these two genes c
ould be induced by the addition of 12-O-tetradecanoylphorbol-13-acetat
e. In a different uncloned neoplastic epithelial cell line (BCl) fluct
uations in the levels of collagenase and transin appeared coordinately
, but coordinate expression was not a feature in cloned lines derived
from BCl. The loss of coordinate regulation of the two matrix metallop
roteinases may relate to the variability in metastatic potential seen
in clonal cell lines.