Rt. Boyd, SEQUENCING AND PROMOTER ANALYSIS OF THE GENOMIC REGION BETWEEN THE RAT NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR BETA-4 AND ALPHA-3 GENES, Journal of neurobiology, 25(8), 1994, pp. 960-973
Nicotinic acetylcholine receptors (nAChRs) found on neurons are compos
ed of ligand binding (alpha) and structural (beta) subunits. Different
combinations of alpha and beta subunits produce nAChR subtypes with d
ifferent pharmacological and ion-conducting properties. Transcriptiona
l regulation may be an important determinant of receptor subtype in a
neuronal population and thus influence transmission through a ganglion
or group of neurons in the CNS by controlling the nAChR subtype(s) pr
esent. In order to understand the transcriptional regulation of neuron
al nAChRs by cell contact and electrical activity, it will be first ne
cessary to identify DNA elements that control the expression of member
s of this family and to identify factors required for the expression o
f these genes. In this report we have begun to examine the 5'-flanking
region of one member of the nAChR family of genes, alpha 3. We have s
equenced the region between the beta 4 and alpha 3 genes and have iden
tified two promoter regions in the beta 4-alpha 3 intergenic region. O
ne region is close to the beta 4 gene downstream of exon 6 and has str
ong promoter activity in both orientations; the other is close to the
start of the alpha 3 gene coding region. A region with putative silenc
er activity is also found near the upstream promoter. This bidirection
al promoter region could be involved in the control of alpha 3 and bet
a 4 gene expression. (C) 1993 John Wiley & Sons, Inc.