DEVELOPMENT OF PACKED CAPILLARY COLUMN ELECTROCHROMATOGRAPHY MASS-SPECTROMETRY

The enhanced performance of electro-osmotically driven high performanc e liquid chromatography (HPLC) using reversed-phase packed capillaries has been evaluated for coupling to mass spectrometers, as an alternat ive to both conventional pressure-driven elution, and to micellar elec trokinetic capillary chromatography for the separation of neutral anal ytes. An off-line comparison of electro-osmotically driven versus pres sure driven chromatography for the separation of an aromatic mixture s hows the superiority of the method over conventional HPLC. Coupling of the technique with continuous-flow fast-atom bombardment for the anal ysis of a steroid mixture is described.