EXPRESSION OF COMPLEMENT C1QB AND C4 MESSENGER-RNAS DURING RAT-BRAIN DEVELOPMENT

This study examined the distribution of complement ClqB and C4 mRNAs d uring rat brain development by northern blot and in situ hybridization . Both Clq and C4 mRNAs were already present at embryonic day 14 (E14) and showed little change in abundance through six weeks postnatal. At E16, ClqB mRNA was present at high abundance in putative microglia/ma crophages in cortical marginal and intermediate zones, and hippocampal anlage, but not in the neurogenic ventricular or sub-ventricular zone s. C4 mRNA had a broadly similar regional distribution, but was presen t at lower abundance in a larger number of cells, putatively neurons. The distribution pattern for ClqB and C4 mRNAs did not change apprecia bly as brain development proceeded. The lower prevalence of C mRNAs in neuroepithelial or subventricular zones suggests an inverse relations hip of C mRNA to cell proliferation. The frequency of apoptotic nuclea r profiles, which was as much as ten-fold higher at P7 vs. E17, did no t correlate anatomically with ClqB or C4 mRNA levels. Thus, the widesp read distribution and consistent presence of each C mRNA during develo pment argues against a role for C in programmed cell death during brai n development. We suggest that Clq and C4 components have novel roles during brain development that may be unrelated to normal cytotoxic act ions of the activated classical C cascade.