PERSISTENT PHYSIOLOGICAL-EFFECTS CAUSED BY A SINGLE PENTYLENETETRAZOL-INDUCED SEIZURE IN NEONATAL RATS

A single seizure was induced by pentylenetetrazol (PTZ; 150 mg/kg i.p. ) in 1-day-old and 21-day-old rats; control littermates were given sal ine (i.p.) injections. In vitro recordings were made in hippocampal sl ices derived from adult (2-3.5-month-old) rats. The population respons es in CA1, CA3 and dentate gyrus (DG) were recorded following double-p ulse stimulation of Schaffer collateral (CA1 stratum radiatum, for CA1 and CA3 recordings) and perforant path (for DG recordings). Paired-pu lse stimuli at an interpulse interval (IPI) of 10-200 ms and intensity of 1.5, 2 or 4 times the stimulus threshold were used. PTZ given on d ay 1 resulted in a highly significant increase in the paired-pulse fac ilitation (PPF) of the population EPSP, but not of the population spik e, in CA1 at all stimulus intensities. In the DG, PPF of both the popu lation EPSP and population spike was found at 1.5 x threshold intensit y. PTZ given on day 21 decreased PPF of the population EPSP and spike in CA1 and had no significant effect in the DG. No significant differe nce was found in CA3 responses after seizures on day 1 or day 21. The slices from seized and control animals were not different in their sti mulus thresholds or response to a single pulse. It is concluded that a single neonatal PTZ-induced seizure had long-lasting physiological co nsequences which depend on the age of seizure. It is suggested that da y-1 PTZ treatment resulted in an increase in presynaptic facilitation in CA1, which was perhaps compensated by a concomitant suppression of spike excitability evoked by the second pulse, resulting in normal PPF of the population spike.