EFFECT OF DAILY REPLACEMENT THERAPY WITH RECOMBINANT BOVINE SOMATOTROPIN ON SOMATOTROPIN, INSULIN-LIKE GROWTH-FACTOR-I, AND ONSET OF PUBERTY IN BEEF HEIFERS IMMUNIZED AGAINST GROWTH HORMONE-RELEASING FACTOR

Two experiments examined whether replacement therapy with recombinantl y derived bovine somatotropin (rbST) would induce puberty in heifers t hat had been actively immunized at 6 mo of age against growth hormone- releasing factor (GRF). Heifers received daily i.m. injections of 25 m g of rbST (Exp. 1, n = 6; Exp. 2, n = 4) or vehicle (VEH; Exp. 1, n = 6; Exp. 2, n = 4) for 56 d. Serum concentrations of somatotropin (ST, nanograms/milliliter) were low in all heifers before first injection i n Exp. 1 (1.56 +/- .04) and 2 (.95 +/- .03). During treatment, serum S T was greater (P < .01) in rbST than in VEH heifers (75.4 +/- 4.8 vs 2 .8 +/- .1 ng/mL, respectively) in both experiments and remained increa sed through d 57 (32.2 +/- 6.4 vs .90 +/- .01 ng/mL). In Exp. 1 and 2, concentrations of serum IGF-I were similar in rbST and VEH heifers be fore treatment, increased (P < .01) 12 h after first rbST, and remaine d increased (P < .01) through d 57 in rbST heifers. Concentrations of serum insulin (INS) and plasma glucose (GLU) were similar (P > .10) in rbST and VEH heifers before first injection (Exp. 1 and 2). Serum INS (micro-units/milliliter) was greater (P < .01) in rbST (61.7 +/- 3.7 and 36.0 +/- 2.4) than in VEH (12.4 +/- 1.6 and 8.1 +/- 1.0) heifers o n d 1 or 2 only, in Exp. 1 and 2, respectively. In Exp. 1, GLU was inc reased (P < .05) by rbST on d 2 through 57, but only on d 1 in Exp. 2. Proportion of heifers pubertal by d 21 tended to be greater (P < .07) in rbST (3 of 6) than in VEH (0 of 5) heifers in Exp. 1, but not in E xp. 2 (1 of 4 vs 1 of 4, respectively). All heifers in Exp. 1 and 50% of the heifers in Exp. 2 attained puberty by d 56. Daily rbST increase d ST, IGF-I, INS, and GLU but did not hasten onset of puberty in heife rs immunized against GRF.