STRATEGIES OF SIGNAL ASSIGNMENTS IN PARAMAGNETIC METALLOPROTEINS - ANNMR INVESTIGATION OF THE THIOCYANATE ADDUCT OF THE COBALT(II)-SUBSTITUTED HUMAN CARBONIC-ANHYDRASE-II

The title protein with MW 30,000 containing high-spin cobalt(II) has b een thoroughly investigated through H-1 NMR spectroscopy with the aid of selectively deuterated amino acids and N-15 enrichment. The aim is that of showing the potentiality of the approach when local informatio n by NMR is needed and the X-ray structure is available. The potential use of the pseudocontact shift is discussed; 90, 200, and 600 MHz spe ctrometers are used to investigate a spherical region at various dista nces from the metal ion. More than 35 signals of protons around cobalt (II) have been assigned. (C) 1994 Academic Press, Inc.