HOMOLOGIES BETWEEN T-CELL RECEPTOR JUNCTIONAL SEQUENCES UNIQUE TO MULTIPLE-SCLEROSIS AND T-CELLS MEDIATING EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS

Citation
M. Allegretta et al., HOMOLOGIES BETWEEN T-CELL RECEPTOR JUNCTIONAL SEQUENCES UNIQUE TO MULTIPLE-SCLEROSIS AND T-CELLS MEDIATING EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS, The Journal of clinical investigation, 94(1), 1994, pp. 105-109
Citations number
34
Categorie Soggetti
Medicine, Research & Experimental
ISSN journal
00219738
Volume
94
Issue
1
Year of publication
1994
Pages
105 - 109
Database
ISI
SICI code
0021-9738(1994)94:1<105:HBTRJS>2.0.ZU;2-T
Abstract
The selection of T cell clones with mutations in the hypoxanthine guan ine phosphoribosyltransferase (hprt) gene has been used to isolate T c ells reactive to myelin basic protein (MBP) in patients with multiple sclerosis (MS). These T cell clones are activated in vivo, and are not found in healthy individuals. The third complementarity determining r egions (CDR3) of the T cell receptor (TCR) alpha and beta chains are t he putative contact sites for peptide fragments of MBP bound in the gr oove of the HLA molecule. The TCR V gene usage and CDR3s of these MBP- reactive hprt(-) T cell clones are homologous to TCRs from other T cel ls relevant to MS, including T cells causing experimental allergic enc ephalomyelitis (EAE) and T cells found in brain lesions and in the cer ebrospinal fluid (CSF) of RIS patients. In vivo activated MBP-reactive T cells in MS patients may be critical in the pathogenesis of MS.